The SAT line (Sanger AB Tübingen) is a combination of two lines, AB and TU. Amanda Rapp and Catherine Wilson in John Postlethwait’s lab created a male doubled haploid AB and a female doubled haploid TU fish (DHTu2 S#18728). Both fish were sequenced to 40x coverage using Illumina GA sequencing technology. In comparison to Zv8, the sequencing yielded over 10 million SNPs, and an Affymetrix custom SNP chip with over 200,000 SNPs was created. Each sequenced fish is homozygous, and they were mated to produce genetically identical and heterozygous F1 hybrids, each having one intact AB and TU chromosome from each parent. These F1s were crossed to produce F2s with one crossover per chromosome. Using MSTmap, this F2 cross was utilised to create a new SAT meiotic map of 140,306 SNPs across 430 F2s. 

the nature of this cross, the SAT line should be mostly devoid of embryonic fatal mutations, since they should have been selected against by the doubling haploid process (any remaining lethal mutations might have been complemented by wild-type maternal mRNA or protein). Similarly, trans-heterozygous lethal alleles in F1s should not exist. Dense genotyping should be able to reconstruct the genome for F2s and future generations.

Tübingen (TU)-

The Tuebingen strain was discovered at a Tuebingen pet shop.

Short, wild-type fins. Sanger’s strain for the zebrafish sequencing project. Before being utilised for mutagenesis and sequencing, it was cleaned up to eliminate embryonic fatal mutations from the background.

Tüpfel long fin (TL)-

Homozygous for the genes leot1 and lofdt2. Obtained from a vendor and maintained by growing mixed eggs from several egg lays of well-laying females. Leot1 is a recessive mutation that causes adult fish spotting, often known as tup. Long fins are caused by a dominant homozygous viable mutation called lofdt2.